Thursday, February 11, 2010

What is the working distance of a microscope?how is it related to produce a clear image of specimen?

(science)help me plsss..What is the working distance of a microscope?how is it related to produce a clear image of specimen?
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Field of View or Field Diameter is very important in microscopy as it is a more meaningful number than ';magnification';. Field diameter is simply the number of millimeters or micrometers you will see in your whole field of view when looking into the eyepiece lens. It is just as if you put a ruler under the microscope and counted the number of lines.





The chart below will tell you (approximately) what to expect when looking through a microscope with varying combinations of eyepiece and objective lenses. As an example (in green below), a dual power stereo microscope with 10X eyepiece lenses and 1X and 3X combinations of objective lenses, would have total powers of 10X and 30X and your field of view would be 20 mm and 6.7 mm respectively.





This means that an object 20 mm (2 cm, or about 3/4 inch) wide would fill up the whole viewing area at 10X and an object about 6.7 mm wide would fill up the whole area at 30X. As you can see, having the highest power may not be best for your particular application. When you move to greater magnifications, you sacrifice field of view.What is the working distance of a microscope?how is it related to produce a clear image of specimen?
It depends on the focal length of the lens itself. Clear image can be obtained if the specimen sitting on the focal length of the lens.

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